Organic cleaning solutions such as as methanol or ethanol should never be used, nor should diffusion chambers be washed with alcohol or autoclaved; as the chambers will be damaged.

New diffusion chambers require an initial washing to remove all traces of machine lubricants. Ultrasonic cleaning with a detergent is also recommended after each use.

Alternatively you could wash with trisodium phosphate detergent and then rinse with excessive volumes of filtered deionized water (i.e. dH2O).

Chambers can be disinfected with 50% bleach, or sterilized with Wescodyne or an ethylene oxide (i.e. ETO) treatment.

Alternatively, cleaning agents such as Ter-g-A-Zyme made by Alconox can be used. This is a concentrated anionic detergent with protease enzymes for manual and ultrasonic cleaning. Excellent for removal of proteinaceous soils, tissue, blood, and body fluids from glassware, metals, plastic, ceramic, porcelain, rubber and fiberglass with no interfering residues.

UV illumination might also work but you should note that the acrylic material itself absorbs UV light so you need to fully "bathe" all of the surfaces with UV for that to be effective.