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There were 5 questions found in this category:
  1. questionThe bridge fittings for my ussing chamber are too long
    The bridge fittings are deliberately cut long to allow you to precisely size them for your setup (excised membrane or culture cup). Simply trim the tip to the correct size, taking into account that the voltage electrode needs to be as close to the membrane as is feasible.Also, note that the meta ...

  2. questionWhat are the differences between round and slotted inserts?
    Round and slotted inserts can be interchanged provided the tissue is large enough. Usually, a slotted insert is employed for a small diameter intestinal section. The extra length increases the surface area while accommodating the small width.

  3. questionWhich Sylgard product is recommended for use with Ussing chambers?
    The most common version used in electrophysiology is Sylgard 184. Several vendors can be found with a Google search of the full name.

  4. questionWhy would I select pins over o-rings in an Ussing System?
    If the basement membrane is strong enough, then pins can be used. It is easier to stretch the membrane over the hole with pins.If the tissue is more fragile, then you must use o-rings. The tissue can be held into place during assembly by placing a small amount of vacuum grease around the o-ring.

  5. questionDon't see your answer? Please submit a question!
    We welcome your questions! Submit a question to us (via email) by clicking here and we'll answer you directly. We'll also anonymously post your question to our FAQ Knowledge Base (KB) to assist other people.If you choose to not communicate via this KB, then please send your questions directly to ...


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