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NaviCyte Horizontal
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There were 7 questions found in this category:
 Can Navicyte chambers be sterilized?
Yes, chambers can be disinfected with 50% bleach, or sterilized with Wescodyne or an ethylene oxide (i.e. ETO) treatment. Alternatively, cleaning agents such as Ter-g-A-Zyme made by Alconox can be used. This is a concentrated anionic detergent with protease enzymes for manual and ultrasonic clea ...
- Can the gas manifold be used with the horizontal diffusion system?
The gas manifold is not normally used with the horizontal chamber. Normally, if a gassed solution needs to be introduced into the horizontal chamber it will first be bubbled into the media, which is then pumped into the basolateral chamber. Since the gas manifold is basically twelve needle valve ...
- How should the Navicyte chambers be cleaned?
Organic cleaning solutions such as as methanol or ethanol should never be used, nor should diffusion chambers be washed with alcohol or autoclaved; as the chambers will be damaged.New diffusion chambers require an initial washing to remove all traces of machine lubricants. Ultrasonic cleaning wi ...
- The bridge fittings for my ussing chamber are too long
The bridge fittings are deliberately cut long to allow you to precisely size them for your setup (excised membrane or culture cup). Simply trim the tip to the correct size, taking into account that the voltage electrode needs to be as close to the membrane as is feasible.Also, note that the meta ...
- What are the differences between round and slotted inserts?
Round and slotted inserts can be interchanged provided the tissue is large enough. Usually, a slotted insert is employed for a small diameter intestinal section. The extra length increases the surface area while accommodating the small width.
- What are the top and bottom volumes of the NaviCyte horizontal ussing chamber?
The volume of the basolateral (lower) compartment is 1.5 ml. The volume of the apical (upper) compartment is 1.5-2.0 ml, depending on if electrodes are used, and if the tissue ring or Snapwell culture cups is used.1.5 ml applies for the case of a tissue ring without electrodes, and 2.0 ml applie ...
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