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There were 13 questions found in this category:
  1. questionCan the leads in the headstage DIN connector be repaired?
    Yes. While we can make a repair at the factory, it is a simple enough task that the user can make a repair on-site.The connector used for most Warner devices (amplifiers, temperature controllers, etc.) are known as DIN type connectors. They can be opened by removing the set-screw on the side and ...

  2. questionCan the SH-27B be used at flow rates less than 2ml/min?
    The SH-27B is designed to work at flow rates of 2-10 ml/min. You can use it at flow rates of less than 2 ml/min, however, the heater is robust and will boil solutions when used at very slow flow rates or in a stopped-flow condition.As an alternative, the SF-28 in-line solution heater is designed ...

  3. questionCan the stainless steel tubing in the inline solution heaters contaminate my perfusate?
    On rare occasions, heavy metal contamination (e.g. Fe) of a solution in contact with stainless steel can be a problem. In such cases we recommend using stretched PE-50 tubing in the SH-27B or SF-28 inline solution heaters. The fact that the stainless steel tubing inside these heaters is a strai ...

  4. questionHow can I exceed the 10 ml/min flow-rate limit for inline solution heaters?
    This can be achieved by using two in-line heaters (e.g., two SH-27B) and a dual channel temperature controller. Basically, split the incoming flow line into two just prior to the in-line heaters (one heater per line) and then rejoin the lines before delivery into the chamber.Another approach wou ...

  5. questionHow can keep perfusate from coming in contact with the stainless steel tubing in an in-line heater?
    The stainless steel tubing in the SF-28 and SH-27B in-line heaters may be lined with PE-90 tubing (1.27 mm OD and 0.86 mm ID). Slightly stretch the PE-90 tubing and thread it through the stainless steel tubing. The PE tubing will relax into it's normal shape over a few minutes and should provide ...

  6. questionHow can the temperature of the perfusate be monitored once it exits an inline solution heater?
    Warner's inline solution heaters (SH-27B and SF-28) and inline solution heater/cooler (SC-20) is supplied with a separate thermistor cable assembly (TA-29) that allows for monitoring the temperature of any point of interest (usually the bath itself).

  7. questionHow can the temperature within an inline solution heater or cooler be monitored?
    Warner's inline solution heaters (e.g., SH-27B, SF-28) and inline solution heater/coolers (e.g., SC-20) contain a built-in feedback thermistor and so do not require a separate thermistor for setting and monitoring the control temperature.A temperature control unit (e.g., TC-324B, TC-344B, CL-10 ...

  8. questionIs it possible for the SH-27B and SF-28 inline solution heaters to overheat under no-flow conditions?
    The SH-27B can overheat. The SF-28 will not. While the SH-27B has a feedback thermistor, under very slow or no-flow conditions it can still overheat. This is because the thermistor is located near to the outflow end of the heater. As a result, this thermistor can cool slightly when the flow is o ...

  9. questionIs it possible to use temperature feedback from the bath to regulate an inline solution heater?
    Unfortunately, no. Feedback in inline solution heaters is derived from thermistors located within the heater body. This prevents oscillations in temperature which can arise when the temperature sensor is distal to the heat source..

  10. questionWhat is the accuracy of the SH-27B and SF-28 inline solution heaters?
    When the TC-324B and TC-344B are used to power either of these inline solution heaters, 37°C can be maintained to within ±0.1°C under the following conditions: a.) the solution temperature at input (nominally 21°C) varies no more than ±10% andb.) the solution flow rate ...

  11. questionWhen is in-line heating preferred over chamber heating?
    Generally speaking, heating a platform alone is a good strategy only for very slow or non-flow conditions. The solution loses most of its heat enroute to the chamber. Under the more common flow conditions of 2-10 ml/min, an inline heater is a better way to deliver heated solution to a chamber.Co ...

  12. questionCan an inline solution heater be cleaned in an ethanol steam sterilizer?
    We dot recommend steam sterilization.During ethanol steam sterilization, the seal on the inline heater may not be tight enough to prevent fluid from reaching the electronics within the heater . Using ethanol, however, to flush the stainless steel tube within the heater body should be sufficient.

  13. questionDon't see your answer? Please submit a question!
    We welcome your questions! Submit a question to us (via email) by clicking here and we'll answer you directly. We'll also anonymously post your question to our FAQ Knowledge Base (KB) to assist other people.If you choose to not communicate via this KB, then please send your questions directly to ...


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