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In-Line Heaters and Coolers
There were 20 questions found in this category:
- Can I use the rapid flow in-line solution heater (SH-27B) for flow rates less than 2ml/min?
The SH-27B is capable of heating from 2-10ml/min and is therefore a more robust heater. As such, it will boil your solutions if you try to go to a stopped-flow condition.The SF-28 in-line solution heater is designed for use from zero flow up to 2ml/min.
- Can the SH-27B be used at flow rates less than 2ml/min?
The SH-27B is designed to work at flow rates of 2-10 ml/min. You can use it at flow rates of less than 2 ml/min, however, the heater is robust and will boil solutions when used at very slow flow rates or in a stopped-flow condition.As an alternative, the SF-28 in-line solution heater is designed ...
- Can the stainless steel tubing in the inline solution heaters contaminate my perfusate?
On rare occasions, heavy metal contamination (e.g. Fe) of a solution in contact with stainless steel can be a problem. In such cases we recommend using stretched PE-50 tubing in the SH-27B or SF-28 inline solution heaters. The fact that the stainless steel tubing inside these heaters is a strai ...
- Can the TC-202A be used to regulate the temperature of Warner's in-line solution heaters, dish heaters and syringe warmers, or Series 20 platforms?
No, since the TC-202A designed for Medical Systems products, it is not compatible with Warner products. The Warner TC-324B/TC-344B, however, has an adapter that makes it compatible with Medical Systems products.
- How can I exceed the 10 ml/min flow-rate limit for inline solution heaters?
This can be achieved by using two in-line heaters (e.g., two SH-27B) and a dual channel temperature controller. Basically, split the incoming flow line into two just prior to the in-line heaters (one heater per line) and then rejoin the lines before delivery into the chamber.Another approach wou ...
- How can keep perfusate from coming in contact with the stainless steel tubing in an in-line heater?
The stainless steel tubing in the SF-28 and SH-27B in-line heaters may be lined with PE-90 tubing (1.27 mm OD and 0.86 mm ID). Slightly stretch the PE-90 tubing and thread it through the stainless steel tubing. The PE tubing will relax into it's normal shape over a few minutes and should provide ...
- How can the temperature of the perfusate be monitored once it exits an inline solution heater?
Warner's inline solution heaters (SH-27B and SF-28) and inline solution heater/cooler (SC-20) is supplied with a separate thermistor cable assembly (TA-29) that allows for monitoring the temperature of any point of interest (usually the bath itself).
- How can the temperature within an inline solution heater or cooler be monitored?
Warner's inline solution heaters (e.g., SH-27B, SF-28) and inline solution heater/coolers (e.g., SC-20) contain a built-in feedback thermistor and so do not require a separate thermistor for setting and monitoring the control temperature.A temperature control unit (e.g., TC-324B, TC-344B, CL-10 ...
- How do I clear a clogged SF-28 or SH-27B inline solution heater?
There are several approaches to take, starting with the most gentle. First, fill the tubing with ethanol and let it soak for a while. If necessary, use some pressure to force ethanol through the tubing. If there is still a partial clog, try to get some dental floss through the tubing and gently ...
- Is it possible for the SH-27B and SF-28 inline solution heaters to overheat under no-flow conditions?
The SH-27B can overheat. The SF-28 will not. While the SH-27B has a feedback thermistor, under very slow or no-flow conditions it can still overheat. This is because the thermistor is located near to the outflow end of the heater. As a result, this thermistor can cool slightly when the flow is o ...
- Is it possible to use temperature feedback from the bath to regulate an inline solution heater?
Unfortunately, no. Feedback in inline solution heaters is derived from thermistors located within the heater body. This prevents oscillations in temperature which can arise when the temperature sensor is distal to the heat source..
- What are the design characterists of the SH-27B and SF-28 inline solution heaters?
The SH-27B is our standard inline solution heater. It is designed to maintain 37 °C in the output solution at flow rates between 2-10 ml/min. The SF-28 is designed for for flow rates between 0-2 ml/min. Note that this device can be safely used even under stopped-flow conditions. Both heaters ...
- What is the accuracy of the SH-27B and SF-28 inline solution heaters?
When the TC-324B and TC-344B are used to power either of these inline solution heaters, 37°C can be maintained to within ±0.1°C under the following conditions: a.) the solution temperature at input (nominally 21°C) varies no more than ±10% andb.) the solution flow rate ...
- What is the difference between the SH-27B and the SF-28?
The SH-27B is our standard inline solution heater. It is designed to provide tight thermal control at flow rates between 2-10 ml/min. The SF-28 is designed for flow rates less than 2 ml/min. An advantage of the SF-28 is that it will also function well in stopped-flow conditions.
- What is the tube size of the inlet and outlet ports of the SH-27B in-line solution heater?
Warner ships this item with a piece of tygon tubing on both ports to act as a bridge. The Tygon tubing is 1/16 ID and 1/8 OD.
- What is the volume of the fluid space in the SH-27B in-line heater?
The fluid volume of the 16 guage, stainless steel tube is 262 ul.
- What will happen if I leave the SH-27B inline solution heater on with no solution in it or with the solution not flowing?
Under these conditions the SH-27B inline solution heater will get very hot and will probably reach its maximum temperature (~60-70°C).
- When is in-line heating preferred over chamber heating?
Generally speaking, heating a platform alone is a good strategy only for very slow or non-flow conditions. The solution loses most of its heat enroute to the chamber. Under the more common flow conditions of 2-10 ml/min, an inline heater is a better way to deliver heated solution to a chamber.Co ...
- Can an inline solution heater be cleaned in an ethanol steam sterilizer?
We dot recommend steam sterilization.During ethanol steam sterilization, the seal on the inline heater may not be tight enough to prevent fluid from reaching the electronics within the heater . Using ethanol, however, to flush the stainless steel tube within the heater body should be sufficient.
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