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Bilayer Training
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There were 4 questions found in this category:
 How can bilayer capacitance be measured using a patch clamp amplifier?
Patch clamp amplifiers do not have intrinsic bilayer capaciatance measurement capability. Therefore, this important measurement must be performed using a simple protocol.In general, a calibrated triangular wave is applied to the membrane and then read the capacitance from the amplitude of the re ...
- Is sodium phosphate dibasic a suitable substitute for sodium phosphate tribasic when cleaning bilayer cups and chambers?
While the tribasic salt (TSP) is the clearly effective, a dibasic salt (DSP) can also work if the concentration is increased slightly. The monobasic salt, however, is pretty much useless. My best recommendation is to not waste your time with the dibasic or monobasic salts and go directly to the ...
- What are some comments for the formation of membranes for beginning bilayerologists?
First, look at page 25 in the BC-535 manual for a brief introduction. As a start, I've had good results using a 5:3 ratio of PE:PC at 30-50 mg/ml in decane. If your lipids came lyophilized, then they will need to be brought up in chloroform to solubilize them. Then, aliquot the desired ratio int ...
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