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RC-30WA
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There were 13 questions found in this category:
 Can the RC-30WA function as a stop-flow device?
The term 'stop-flow' assumes there will be a perfusion system in place, but the flow is intended to stop periodically. First, we've never specifically tested the chamber as a stop-flow device and so have not quantitated how the solution comes to rest. We have made many informal measurements with ...
- How can I generate and maintain a hypoxic solution for delivery to a chamber?
This is normally achieved by bubbling an O2-free gas through the solution until the O2 load has been depleted. Keep in mind, however, that PE, Tygon, and Teflon tubing are all gas permeable, and therefore will expose your perfusing solutions to O2 during transit.One option to address this is to ...
- Is the RC-30HV tested at different flow rates and if so, what is the highest flow at which the liquid stays in the channel?
While we have measured the flow rate in the RC-30 to upwards of 20 ml/min, these chambers typically support flow rates from 0.1 to 2.0 ml/min. This lower flow range is not due to physical limitations of the chambers but is more reflective of the experimental conditions.. Many researchers speak o ...
- What bath geometries are available with the RC-30 series confocal imaging chambers?
Pre-cut gaskets are supplied with the RC-30 series chambers; one cut wide to allow full use of the aperture window and a narrow, slotted version for fast solution exchange. A blank gasket, that has not been pre-cut, is also provided to allow for a user-defined geometry.
- What do I do if my RC-30 confocal imaging chamber leaks?
The gaskets that are used with the RC-30 confocal imaging chamber come in three thicknesses: 150, 250 and 375 um. Leaking is not uncommon when only a single150 um gasket is used. This is because the thinnest gasket allows little compression. We suggest using either a 250 um gasket or two 150 um ...
- What is the bath volume of the RC-30 Series gaskets?
Gaskets come in 3 varieties, slotted, wide-bath, and blank. For a 250 um thick gasket, the slotted and wide-bath hold 26 and 133 ul, respectively. Since the blank is designed to allow the creation of a custom bath geometry, you'll need to calculate that volume independently.
- What thickness gaskets are available for use with the RC-30 series confocal imaging chambers?
Gaskets for the RC-30 series chambers are available in three thicknesses; 100uM, 250uM and 375uM.
- Where should the thermistor be placed in the RC-30HV chamber?
Because the RC-30HV is a closed bath chamber, it is necessary to put the small, monitor thermistor into the chamber's outflow stream. One method is to put a small tee fitting in the outflow line and insert the thermistor into the open port. Seal around the thermistor with silicone grease or syl ...
- Are the gaskets used in the RC-30 Chambers easy to cut?
The gaskets are very easy to cut as long as you work on a hard surface and use a sharp blade.
- Can a CC-28 thermistor be repaired?
Yes, both the copper-colored monitor and white feedback thermistors can be replaced. The replacement parts come with instructions for attaching to the CC-28 cable. TS-60P 64-0269 White feedback thermistor TS-70B 64-0270 Copper-colored monitor thermistor
- Can a PDMS microfluidic device be used in lieu of gaskets with an RC-30 Chamber?
A thin layer of PDMS works very well as long as the upper surface is kept flat during fabrication. (We've tried it!) This statement assumes the chamber is built onto the bottom coverslip.
- Don't see your answer? Please submit a question!
We welcome your questions! Submit a question to us (via email) by clicking here and we'll answer you directly. We'll also anonymously post your question to our FAQ Knowledge Base (KB) to assist other people.If you choose to not communicate via this KB, then please send your questions directly to ...
- What is the thickness of the coverglass you carry?
We carry coverglass in three thicknesses: #1, #1.5, and #2Generally speaking, glass thickness is expressed as a range to allow for manufacturing variance and our glass adheres to industry standards.#1 = 0.13 - 0.17 mm (130-170 µm)#1.5 = 0.16 - 0.19 mm (160-190 µm) #2 = 0.17 - 0.25 mm ...
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