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RC-21BRFS
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There were 12 questions found in this category:
 Can Warner Series 20 Perfusion Chambers be used with #0 thickness Cover Glass
Nothing speaks against the use of #0 thickness glass coverslips with our Series 20 chambers. However, you might want to use a P- or PH- platform with screw-clamps rather than a PM- platform with magnetic clamps. The screw- clamps will allow you to adjust the clamp pressure. Be careful not to br ...
- How can a ground wire be attached to a chamber that doesn't have an agar bridge?
A silver-silver chloride ground electrode can be placed directly into the suction well downstream from the sample. Be aware, however, that if you do not continuously perfuse the sample, the silver wire may be able to contaminate the bath.
- How can I generate and maintain a hypoxic solution for delivery to a chamber?
This is normally achieved by bubbling an O2-free gas through the solution until the O2 load has been depleted. Keep in mind, however, that PE, Tygon, and Teflon tubing are all gas permeable, and therefore will expose your perfusing solutions to O2 during transit.One option to address this is to ...
- How do I clean the Warner chambers?
We recommend using our TSP wash. A reference to this procedure can be found below
- What are the electrodes in the field stimulation chambers composed of?
The electrodes in our field stimulation chambers are platinum.
- What length of electrode is exposed to the bath in your field stimulation chambers?
Chamber Exposed Electrode (on each side of bath)RC-21BRFS 12mmRC-27NE 25mmRC-37FS 19mmRC-37WS 13mmRC-47FSLP 15mm
- What type of grease is used for sealing the gaskets and coverglass on RC Series chambers?
The grease we recommend is the Dow 111 silicone grease that is normally used with vacuum pump tubing line connections. It is also used to seal glass dessicators. We sell two products that contain a tube of this grease. Order number 64-0275 is simply a tube of Dow 111 silicone, while order number ...
- Are electrodes in field stimulation chambers exposed to the bath?
The electrodes are exposed to the bath in order to insure the cells are exposed to significant field stimulation.
- At what bath height should electrodes be placed in field stimulation chambers?
The electrodes in the field stimulation chambers are placed at the bottom of the bath to allow close proximity to the cells. This insures that cells are exposed to the maximum field strength.
- Can a CC-28 thermistor be repaired?
Yes, both the copper-colored monitor and white feedback thermistors can be replaced. The replacement parts come with instructions for attaching to the CC-28 cable. TS-60P 64-0269 White feedback thermistor TS-70B 64-0270 Copper-colored monitor thermistor
- Don't see your answer? Please submit a question!
We welcome your questions! Submit a question to us (via email) by clicking here and we'll answer you directly. We'll also anonymously post your question to our FAQ Knowledge Base (KB) to assist other people.If you choose to not communicate via this KB, then please send your questions directly to ...
- What is the thickness of the coverglass you carry?
We carry coverglass in three thicknesses: #1, #1.5, and #2Generally speaking, glass thickness is expressed as a range to allow for manufacturing variance and our glass adheres to industry standards.#1 = 0.13 - 0.17 mm (130-170 µm)#1.5 = 0.16 - 0.19 mm (160-190 µm) #2 = 0.17 - 0.25 mm ...
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